Embedding in Butyl-Methyl-Methacrylate

This resin sysetm is ideal for immunocytochemistry because the embedment is removable (to a large extent) after sectioning by means of a brief incubation in acetone. Provided that DTT is added to the resin, the preservation of antigenicity is outstanding.

The resin system is also very easy to section.

Download PDF of protocol for the roots of Arabidopsis thaliana but applicable to other samples

Please contact me if you have specific questions, or with comments on your results, good or bad. baskin@bio.umass.edu