Caveat re: Intensities.
Antibodies were diluted sufficiently to produce median intensities not
more than 100-fold higher than autofluorescence. Percentage saturation
for each antibody is not provided. Until the saturation data are provided,
intensities cannot be
meaningfully compared between different antibodies.
Methods.
Graphs produced with the
WinMDI freeware were copied to the clipboard
and pasted into a composite image in
LviewPro shareware, which was then used to add text labels.
: Ask class to predict graphic before projecting it.
Splenocytes