Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name 03060cfi.htm
Student name  Ryan Benson
Cell type  Splenocyte Cells in scatter gate as % of total events  62

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  TCR  FITC
1  IgM   PE
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1  1  58  3.1  025  2.5  0.6
 FL1  2  41  74  025  2.2  72
FL2   1  47  4.6  025  2.5  2.1
 FL2  2  50  150  025  2.2  140
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.