Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
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Disk file name |
54203054 |
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Student name |
Mulualem E. Tilahun |
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Cell type |
Thymocyte |
Cells in scatter gate as % of total events |
60% |
SAMPLE PREPARATION
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Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
|
1 |
Anti CD4 |
None-Conjugated |
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2 |
Anti IgG Rat |
FITC-Conjugated |
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3 |
Anti CD8 |
PE-Conjuated |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
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Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
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|
Fl 1 |
1 |
1.5% |
4.0 |
54203029 |
2.0 |
2.0 |
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|
2 |
98.5% |
41 |
54203029 |
2.0 |
39 |
|
FL2 |
1 |
8% |
3.2 |
54203029 |
2.0 |
1.2 |
|
|
2 |
92% |
88 |
54203029 |
2.0 |
86 |
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*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.