Immunology Lab 542
FACScan Fluorescence Intensities from
Histograms
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Disk file name |
54203053 |
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Student name |
Maura |
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Cell type |
Splenocytes |
Cells in scatter gate as % of total events |
59% |
SAMPLE PREPARATION
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Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
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1 |
IgM |
PE-conjugated |
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1 |
MHC-1 |
None |
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2 |
IgG |
FITC-conjugated |
RESULTS
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FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI) |
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Peak No.§ |
Peak: % of (Events in Gate) |
Disk File number |
Fluorescence Intensity (FI) |
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FL1 |
----- |
---------- |
184.3 |
026 |
2.5 |
180 |
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FL2 |
1 |
40% |
3.9 |
026 |
2.2 |
1.7 |
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FL2 |
2 |
49% |
132.2 |
026 |
2.2 |
130 |
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*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.