Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  54203050
Student name Maria Lalimarmo 
Cell type  Thymocytes Cells in scatter gate as % of total events  69%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  CD8  none
 2  Polyclonal Goat  anti-rat Fluorochrome (FITC) 
 3  CD4  Phycoerythrin (PE)

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1 M1  9%   6  29  2.0  4
 FL1  M2  91%  129  29  2.0  127
 FL2  M1  100%  25  29  2.0  23
             
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.