Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  54203044
Student name  Jessica Porter
Cell type  Splenocytes Cells in scatter gate as % of total events  57%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
1 IgM  PE 
1  CD8  none 
2   IgG, rat  FITC

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1 M1 96%  3.2  026  2.5  0.7 
FL1  M2  4%  1.3 x 102 026  2.5  1.3 x 102
FL2 M1  44% 4.6  025  2.2  2.4 
FL2  M2  56%  1.8 x 102 025  2.2  1.8 x 102
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.