Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
|
Disk file name |
01017 |
||
|
Student name |
Craig Gassman |
||
|
Cell type |
Thymocytes |
Cells in scatter gate as % of total events |
65% |
SAMPLE PREPARATION
|
Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
|
1 |
Thy-1 |
FITC |
|
2 |
IgG |
|
|
3 |
CD4 |
PE |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
||
|
Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
|||
|
1 |
1 |
0.4% |
17 |
029 |
2.0 |
15 |
|
1 |
2 |
3% |
49 |
029 |
2.0 |
47 |
|
1 |
3 |
95% |
250 |
029 |
2.0 |
248 |
|
|
|
|
|
|
|
|
|
2 |
1 |
7% |
50 |
029 |
2.0 |
48 |
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.