Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02045
Student name  Diana
Cell type  Splenocytes Cells in scatter gate as % of total events  74%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  TCR  FITC
 1  IgM  PE
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1  1  63%  4.6  030  3.0  1.6
FL1  2  37%  67  030  3.1  64
 FL2  1  45%  6.5  030  3.0  3.5
 FL2  2  54%  135  030  3.1  131
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.