Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  542-02-043.htm
Student name  Enobong Etteh
Cell type  Thymocyte Cells in scatter gate as % of total events  65 %

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1 CD 11a  
 2  #A  FITC conjugated goat anti-rat IgG
 3   CD 4  PE

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1  M 1  4 %  16   025  2.2  14
 FL1   M 2  91 %  42   025  2.2 40
FL2  M 1  9 %  23   025  2.5  21
FL 2  M 2  83 %  139  025  2.5 137
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.