Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02042
Student name  Martha C. Escobar
Cell type Splenocytes  Cells in scatter gate as % of total events  73%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  TCR & CD4  FITC, PE 
     
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1 1   65%  4  030  3.0 1 
 FL1  2  35%  72  030  3.0  69
 FL2 1   78%  5  030  3.1  2
 FL2 2   22%  101  030  3.1  98
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.