Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name   #30-54202040
Student name  Chrono K. Lee 
Cell type  thymocytes  Cells in scatter gate as % of total events  68%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  CD4 Rat IgG2a, TCR Hamster IgG  PE-conjugate, FITC-conjugated
     
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1   1  19%  77  026 2.2   75
   2  78%  10  026  2.2   7.8
FL2   1   93%   98  026  2.6   95
   2   5%   7.9  026  2.6   5.3
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.