Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02 037
Student name  Romanus (Analysis by Eric)
Cell type  Splenocytes Cells in scatter gate as % of total events  70%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  CD4 (rat)  
 1  PE-IgM (goat)  PE
 2  rat IgG (goat)  FITC

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 1  1  77%  4.6  030  3.0   1.6 
1   2  22%  66   030  3.0    63 
 2  1  45%  5.2   030  3.1   2.1 
 2  2  55%  121   030      3.1   118 
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.