Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02034
Student name  Doan
Cell type Spenlocyte  Cells in scatter gate as % of total events 71% 

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1 Cd11a and IgM none and PE-conjugated
 2 Goat anti-rat IgG  FITC-conjugated
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1 1   0%  1.8  030  3.0 -1.2
 FL1  2  99.9%  25.7  030  3.0  22.7
 FL2  1  45%  5.5  030  3.1  2.4
FL2  2   54%  161.1  030  3.1 158.0
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.