Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02032
Student name Maynard, L. 
Cell type Splenocytes  Cells in scatter gate as % of total events  69%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  IgM PE 
2   CD8 FITC 
     

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 1  1  10%  136  030  3  133
   2  88%  4  030  3  1
 2  1  55%  132  030  3  129
   2  44%  5  030  3  2
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.