Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02022, using data from 01017
Student name  Emily Brand
Cell type  Thymocytes Cells in scatter gate as % of total events  60 %

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  Thy-1 (FL1)  FITC
 2  Goat anti-mouse  n/a
 3  CD4 (FL2)  PE

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 1  1 1 %  21  54201017 2.8  18
1  2  99 %  250    2.8  247
 2 1   1 %  10    2.3  8
 2  2 99%   153    2.3  151
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.