Equipment:

  UV-capable spectrophotometers (at least 2).
  96-well plate absorbance "ELISA" reader(s).
  Two or 3 computers with Eric's "SEM" program.

Materials:

  Per Class:
    About 200 test tubes, 12-15 ml capacity. (In 2001, students had
			trouble mixing dilutions in smaller tubes.)

  Per Bench:
    2 (or at least one) vortexers.
    Two squirt bottles of distilled water.
    Two sets of four 1.5 ml cuvettes (3 UV + 1 Vis per set, UNMARKED!)
    Can of five ml pipets.
    Can of ten ml pipets.
    Two to four P200 pipeters.
    Two to four P1000 pipeters.
    Blue and yellow tips.

Above: supplies/instruments to be provided/operated by TA's.
Below: reagents and plates will be provided by Eric.

    "Unknown IgG" 20 ml
    "Unknown BSA" 20 ml

  Per Pair:
    One rigid, flat-bottomed 96-well plate.
    NP Stock, 2 ml.
    Diluent/Blank, 60 ml.

Diluent (10 mM phosphate, pH 9), make 1.5 liters. Dissolve 4.0 g Na₂HPO₄^.7H₂O (FW 268) in 1.5 liters water. Not necessary to check pH; it will be close to 9.

NP Stock: need about 30 ml, so make 40 ml. Dissolve 15 milligrams of p-nitrophenol (FW 139) in 40 ml 10-mM-phosphate, pH 9. A₄₀₅ of a 1/37 dilution should be checked and should be close to 0.9.

Stock tryptophan solution for fake proteins, 0.71 mM. In 100 ml pH 9 10-mM-phosphate, dissolve 14 milligrams L-tryptophan (FW 204).

Unknown IgG (fake): need 100 ml tryptophan at 0.071 mM = 71 micromolar. Add 10 ml 0.71 mM tryptophan stock to 90 ml pH 9 phosphate. A₂₈₀ should be checked and should be close to 0.40.

Unknown BSA (fake): need 100 ml tryptophan at 0.035 mM = 35 micromolar. Add 5 ml 0.71 mM tryptophan stock to 95 ml pH 9 phosphate. A₂₈₀ should be checked and should be close to 0.20.