2001 Immunology Lab Syllabus -- Microbiology 542
Eric Martz, emartz@microbio.umass.edu, Ofc: Fernald 209, 545-2325 - Lab: Morrill IVN10, 577-3103.
Tues/Thurs 2:30-5:30 Morrill IV North 301
Computer Lab: Stockbridge 301 (Environmental Sciences)
The class web site has information on grades, flow cytometry, and other important resources. www.bio.umass.edu/immunology/mic542hp.htm 
Date     Topic                                                          DUE

Tu 1/30 Introduction, safety, space assignment
Th 2/1  CI1: Microscope alignment, cell counting, uncertainty
          (CI = Cellular Immunology)

Tu 2/6  FC1: Flow cytometry (FC): introduction and planning assignment
Th 2/8  CI2: Blood leukocytes, lymphoid histology and immunopathology

Tu 2/13 CI3: Mouse lymphoid system dissection, lymphocyte prep'n       FC Plan Due
Th 2/15 MV1: Visualization of ImmunoMolecules - Stockbridge 301

[Tu 2/20 = Monday class schedule]
Th 2/22 FC2: Cell staining

Tu 2/27 FC3: Data acquisition; QUIZ
Th 3/1  FC4: Computer data analysis - Stockbridge 301

Tu 3/6  FC5: Computer data analysis - Stockbridge 301
Th 3/8  FC6: Computer data comparison, work on reports - Stockbridge 301

Tu 3/13 Ig1: Immunoglobulin (Ig): salt precipitation
Th 3/15 Ig2: Purification on DEAE columns

[3/20, 3/22 = Spring Break]

Tu 3/27 FC7: Overview of class results                                 FC Report Due
Th 3/29 Ig3: Spectrophotometry: A280 std curve; pipeting; uncertainty   

Tu 4/3  Ig4: DEAE fractions: A280, pooling, concentration               
Th 4/5  Ig5: Fragmentation by pepsin

Tu 4/10 Ig6: Sephadex G150: pour columns, determine void volume
Th 4/12 Ig7: Sephadex G150 purif of F(ab')2: run, pool fxns & SDS       

Tu 4/17 Ig8: Pour polyacrylamide gels (PAGE)                            
Th 4/19 Ig9: Run, stain, and blot PAGE                                  

Tu 4/24 Ig10: Develop western blots
Th 4/26 Antibodies: a clinical perspective §

Tu 5/1  Ig11: Analyze gel results, work on reports
Th 5/3  ELISA1: theory and planning                                   Ig Report Due

Tu 5/8  ELISA2: standard curve                                        


Th 5/10 ELISA3: unknowns, data analysis                               


Tu 5/15 Review for Final Exam                                         Notebooks Due
Day/time to be announced:
                *** FINAL EXAMINATION ***
  
§ Guest instructor Chester Andrzejewski, M.D., Ph.D., Medical Director,
Transfusion Medicine Service, Baystate Medical Center, Springfield MA.